Enzyme/Nanocopper Hybrid Nanozymes: Modulating Enzyme-like Activity by the Protein Structure for Biosensing and Tumor Catalytic Therapy

Artificial enzymes with modulated enzyme-mimicking activities of natural systems represent a challenge in catalytic applications. Here, we show the creation artificial Cu metalloenzymes based on generation nanoparticles an enzyme matrix. Different were used, and structural differences between especially influenced controlled size environment that surrounds them. Herein, demonstrated oxidase-like activity these copper nanozymes was rationally by used as scaffold, special role nanoparticle their environment. In this sense, nanocopper hybrids have confirmed ability to mimic unique enzymatic completely different from such tyrosinase-like or Fenton catalyst, which has extremely higher stability than mushroom tyrosinase. More interestingly, oxidoreductase-like cooperatively synergistic effect improving catalase (no peroxidase activity). Additionally, novel dual (metallic activity) nanozyme made highly improved catechol-like interesting for design 3,4-dihydroxy-l-phenylalanine (l-DOPA) biosensor detection These also showed cytotoxic against tumor cells, biocatalytic therapy.